Abstract: Chrysomela populi is a typical leaf beetle feeding on mainly leaves of aspen trees, and a major pest for forestry production. Morphological studies on Chrysomela populi, or even on chrysomelid beetles, are surprisingly scarce. In this investigation, Chrysomela populi adults were used for x-ray micro-computed tomography (micro-CT). Before they were scanned with a Skyscan 1172 micro-CT (beam strength: 40 keV), the adult samples were dehydrated with ethanol and dried to the critical point. The scanned image sequence stack includes 1,554 images with a resolution of 1.04 µm, totaling a data volume of 18.7 GB. The image quality is clear and there are no annular pseudomonas. The tissue in the head is also clear and intact; muscles, bones, digestive tracts, and even nervous systems are clearly visible. The scanned image sequence stack could be used for 3D reconstruction and anatomical study of the adult head of Chrysomela populi. This dataset provides basic data for the study of beetle head morphology and anatomy.
Keywords: micro-CT, 2D image; image stack; 3D reconstruction